Ecology and ecophysiology of invasive and native decapod crabs in the southern North Sea

The Asian shore crab Hemigrapsus sanguineus is native to the coasts of China, Korea and Japan. It first invaded the U.S. east coast in the late 1980s and its present range extends from North Carolina to Maine. It was detected in France and the Netherlands in 1999. Nowadays, H. sanguineus is fully established at the European Atlantic coast. It can be found from the Contentin Peninsula, France to the German Wadden Sea, including the rocky island of Helgoland. In the U.S. and in Europe, H. sanguineus shares habitats with the native European green crab Carcinus maenas and competitive interactions between both are suggested. Thus, the main aims of this thesis were 1) to evaluate the status of H. sanguineus and C. maenas around Helgoland in relation to wave action, 2) to compare the respiratory metabolism and energy demand of both species, and 3) to assess their energy storage capacities and dietary preferences

YOUMARES – A Conference from and for YOUng MARine RESearchers

YOUMARES is an annual early-career scientist conference series. It is an initiative of the German Society for Marine Research (DGM) and takes place in changing cities of northern Germany. The conference series is organized in a bottom-up structure: from and for YOUng MARine RESearchers. In this chapter, we describe the concept of YOUMARES together with its historical development from a single-person initiative to a conference venue of about 200 participants. Furthermore, the three authors added some personals experiences and insights, what YOUMARES means to them

YOUMARES 8 – Oceans Across Boundaries: Learning from each other

This open access book presents the proceedings volume of the YOUMARES 8 conference, which took place in Kiel, Germany, in September 2017, supported by the German Association for Marine Sciences (DGM). The YOUMARES conference series is entirely bottom-up organized by and for YOUng MARine RESearchers. Qualified early career scientists moderated the scientific sessions during the conference and provided literature reviews on aspects of their research field. These reviews and the presenters’ conference abstracts are compiled here. Thus, this book discusses highly topical fields of marine research and aims to act as a source of knowledge and inspiration for further reading and research

YOUMARES 9 - The Oceans: Our Research, Our Future

This open access book summarizes peer-reviewed articles and the abstracts of oral and poster presentations given during the YOUMARES 9 conference which took place in Oldenburg, Germany, in September 2018. The aims of this book are to summarize state-of-the-art knowledge in marine sciences and to inspire scientists of all career stages in the development of further research. These conferences are organized by and for young marine researchers. Qualified early-career researchers, who moderated topical sessions during the conference, contributed literature reviews on specific topics within their research field

Trophic ecophysiology of the native green shore crab, Carcinus maenas, and the invasive Asian shore crab, Hemigrapsus sanguineus, in the rocky intertidal of Helgoland (North Sea)

The brachyuran crabs Carcinus maenas and Hemigrapsus sanguineus belong to the most ´successful´ invaders along the oceans coasts. In 2009, H. sanguineus appeared at the rocky intertidal of the island of Helgoland in the North Sea, where it encounters the native Green shore crab, C. maenas. H. sanguineus established a self-sustaining population, approaching in numbers and biomass that of C. maenas. Both species are considered to be opportunistic omnivores with variable food preferences and, thus, are potential competitors for food. To evaluate the intrinsic properties of either species to utilize food, we analyzed their stomach content, the morphology of the gastric mills, which shred the ingested food, the activities of digestive enzymes during a seasonal cycle, and the stable isotope ratios. A huge share of the stomach contents was macerated and, thus, could not be identified. The shares of animal food and algae food were almost equal in C. maenas but algae food dominated over animal food in H. sanguineus. The gastric mill of C. maenas shows blunt medial tooth and rounded lateral teeth, which indicates efficient grinding of a carnivorous diet. In contrast, the gastric mill of H. sanguineus shows sharp ridges, which facilitate cutting of algal food. The activities of the proteolytic enzymes trypsin and leucine-aminopeptidase were almost equal in both species with slightly higher activities in C. maenas in summer. The activities of the carbohydrases laminarinase and amylase dominated in H. sanguineus during all seasons. Stable isotope ratios indicate a higher degree of carnivory in C. maenas. The morphological and biochemical features indicate that C. maenas is better suited to utilize animal food and H. sanguineus algal food. Upon scarcity of animal food or severe competition with C. maenas, H. sanguineus may be able to increase the amount of algal food and to utilize it efficiently

Dietary preferences of brachyuran crabs from Taiwan for marine or terrestrial food sources: evidence based on fatty acid trophic markers

Background Trophic interactions are key processes, which determine the ecological function and performance of organisms. Many decapod crustaceans feed on plant material as a source for essential nutrients, e.g. polyunsaturated fatty acids. Strictly herbivorous feeding appears only occasionally in marine decapods but is common in land crabs. To verify food preferences and to establish trophic markers, we studied the lipid and fatty acid composition of the midgut glands of two marine crab species (Grapsus albolineatus and Percnon affine), one semi-terrestrial species (Orisarma intermedium, formerly Sesarmops intermedius), and one terrestrial species (Geothelphusa albogilva) from Taiwan. Results All species showed a wide span of total lipid levels ranging from 4 to 42% of the dry mass (%DM) in the marine P. affine and from 3 to 25%DM in the terrestrial G. albogilva. Triacylglycerols (TAG) were the major storage lipid compound. The fatty acids 16:0, 18:1(n-9), and 20:4(n-6) prevailed in all species. Essential fatty acids such as 20:4(n-6) originated from the diet. Terrestrial species also showed relatively high amounts of 18:2(n-6), which is a trophic marker for vascular plants. The fatty acid compositions of the four species allow to clearly distinguish between marine and terrestrial herbivorous feeding due to significantly different amounts of 16:0, 18:1(n-9), and 18:2(n-6). Conclusions Based on the fatty acid composition, marine/terrestrial herbivory indices were defined and compared with regard to their resolution and differentiating capacity. These indices can help to reveal trophic preferences of unexplored species, particularly in habitats of border regions like mangrove intertidal flats and estuaries

Population development of the invasive crab Hemigrapsus sanguineus (De Haan, 1853) and its potential native competitor Carcinus maenas (Linnaeus, 1758) at Helgoland (North Sea) between 2009 and 2014

The Asian shore crab Hemigrapsus sanguineus (De Haan, 1853) has recently established populations in the North Sea and now occurs within the native ranges of the green crab Carcinus maenas (Linnaeus, 1758). To determine potential competitive effects and to assess the progress of the invasion, species-specific population characteristics (numerical abundances, biomasses, and size distributions) of the two species around the island of Helgoland (German Bight, southern North Sea) were compared for surveys conducted in 2009 and 2014. Sampling sites were chosen based on accessibility and differed in their topography and wave exposure, which allowed testing for the influence of these factors on the establishment success of H. sanguineus. The numerical abundance and biomass of H. sanguineus increased markedly and approached those of C. maenas in 2014. At a sheltered site, H. sanguineus even outnumbered C. maenas, whereas the converse was observed at a site exposed to strong winds and waves. Although such contrasting abundance patterns between the native and the introduced shore crab may be the result of direct interference, the dominance of H. sanguineus at the sheltered site may also be explained by enhanced larval settling rates caused by odors of conspecifics. The results suggest that the invasion of H. sanguineus has not yet reached its equilibrium, and population abundances in the North Sea are expected to further increase in the future

Exploring larval phenology as predictor for range expansion in an invasive species

Predicting range expansion of invasive species is one of the key challenges in ecology. We modelled the phenological window for successful larval release and development (WLR) in order to predict poleward expansion of the invasive crab Hemigrapsus sanguineus along the Atlantic coast of North America and north Europe. WLR quantifies the number of opportunities (in days) when larval release leads to a successful completion of the larval phase; WLR depends on the effects of temperature on the duration of larval development and survival. Successful larval development is a necessary requirement for the establishment of self‐persistent local populations. WLR was computed from a mechanistic model, based on in situ temperature time series and a laboratory–calibrated curve predicting duration of larval development from temperature. As a validation step, we checked that model predictions of the time of larval settlement matched observations from the field for our local population (Helgoland, North Sea). We then applied our model to the North American shores because larvae from our European population showed, in the laboratory, similar responses to temperature to those of a North American population. WLR correctly predicted the northern distribution limit in North American shores, where the poleward expansion of H. sanguineus appear to have stalled (as of 2015). For north Europe, where H. sanguineus is a recent invader, WLR predicted ample room for poleward expansion towards NE England and S Norway. We also explored the importance of year‐to‐year variation in temperature for WLR and potential expansion: variations in WLR highlighted the role of heat waves as likely promoters of recruitment subsidising sink populations located at the distribution limits. Overall, phenological windows may be used as a part of a warning system enabling more targeted programs for monitoring

Seasonal lipid storage and dietary preferences of native European versus invasive Asian shore crabs

The invasive Asian shore crab Hemigrapsus sanguineus and the native European green crab Carcinus maenas share intertidal habitats along European North Atlantic shores and may compete for food. We evaluated the energy-storing capacities of the 2 species and determined their dietary preferences by means of lipid analysis and fatty acid trophic marker indices. Specimens of both sexes and various sizes were sampled in the rocky intertidal of the island of Helgoland (North Sea) in April, June, August, and October 2015. Total lipids of the midgut glands were significantly higher in H. sanguineus than in C. maenas and followed a distinct seasonal cycle in both sexes (ca. 20−50% of dry mass, DM). The lower lipid contents of C. maenas (ca. 20% of DM) remained at a similar level throughout the seasons. The seasonal differences in the females of H. sanguineus may be due to higher reproductive output and, consequently, lipid turnover, but remain unexplained in males. Trophic indices for Bacillariophyceae, Chlorophyta, and especially Phaeophyceae were higher in H. sanguineus than in C. maenas, suggesting a higher degree of herbivory of the invader. In contrast, the Rhodophyta index was higher in C. maenas. Thus, competition for food between the 2 species will probably be low in habitats rich in macroalgae. The ability of H. sanguineus to utilize mainly energy-poor algae but accumulate high-energy reserves may be an advantage for successfully establishing persistent populations in new habitats